Journal: International Journal of Molecular Sciences

Article Title: High-Content Imaging and Machine Learning Classify Phenotypical Change in Coronary Artery Endothelial Cells Caused by BPS

doi: 10.3390/ijms27073259

Figure Lengend Snippet: Representative high-content microscopy images of human coronary artery endothelial cells (HCAEC) exposed to vehicle control (CTRL) or 0.1 µM Bisphenol S (BPS) for 96 h and stained using the PhenoVue Cell Painting assay. For each condition, a representative field acquired at 40× magnification and a higher-magnification inset are shown. Rows correspond to the individual fluorescence channels: Hoechst 33342 (nuclei), PhenoVue Fluor 488 Concanavalin A (endoplasmic reticulum and intracellular membranes), PhenoVue 512 nucleic acid stain (RNA/nucleoli), PhenoVue Fluor 555 wheat germ agglutinin (plasma membrane), PhenoVue 641 mitochondrial stain (mitochondria), and the merged image. White boxes represent the part of the image used for the related inset. Scale bar: 50 µm, 40× objective.

Article Snippet: Primary human coronary artery endothelial cells (HCAEC; ATCC ® PCS-100-020TM, Innovation, VA, USA) were cultured according to the supplier’s recommendations.

Techniques: Microscopy, Control, Staining, Fluorescence, Clinical Proteomics, Membrane

Journal: ACS applied materials & interfaces

Article Title: Extracellular Vesicles Enhance the Remodeling of Cell-Free Silk Vascular Scaffolds in Rat Aortae.

doi: 10.1021/acsami.0c06609

Figure Lengend Snippet: Figure 2: A: Proliferation and B: migration of smooth muscle cells (SMCs) and endothelial cells (ECs) when exposed to extracellular vesicle (EV) based treatments. BM: Basal media; PBS: Phosphate buffered saline; EV50: 50 μl of EV isolate; EV150: 150 μl of EV isolate; SBM: Supplemented basal media. * represents p < 0.05, ** represents p < 0.005 and *** represents p < 0.0001.

Article Snippet: 22 Human coronary artery endothelial cells (ECs) were obtained from Cell Applications 23 (#300-05a), cultured in supplemented basal media (#212K-500, Cell Applications 24 Inc, SBM) and used at passage 4.

Techniques: Migration, Saline

Journal: Journal of Translational Medicine

Article Title: Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic

doi: 10.1186/s12967-024-05120-y

Figure Lengend Snippet: QA improved TMAO-induced inflammatory lesions and endothelial dysfunction in HCAECs. (A) CCK-8 was applied to detect the toxicity of QA on HCAECs. (B) CCK-8 was used to detect HCAECs proliferation. (C) The expression of COX-2, IL-6, E-selectin, ICAM-1, HMGB1 was detected by RT-qPCR. (D) The expression of p-P65, p-MAPK14 protein was detected by western blot. (E) HMGB1 levels were detected by ELISA. (F) The expression of ZO-2, VE-Cadherin and Occludin were detected by western blot. * P < 0.05 vs. Control, # P < 0.05 vs. TMAO

Article Snippet: To investigate the cytotoxicity of QA, human coronary artery endothelial cells (HCAECs, HUM-iCell-c006, iCell) were treated with 1, 2.5, 5, 10 and 20 μM QA.

Techniques: CCK-8 Assay, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 24 h after irradiation. The response of various inflammatory markers at 24 h after 0.1 and 5 Gy irradiation is represented in (A-I) TICAE cells and (J-R) TIME cells, normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 72 h after irradiation. The response of various inflammatory markers at 72 h after 0.1 and 5 Gy irradiation is presented in (A-I) TICAE cells and (J-R) TIME cells. Data were normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting